To achieve the highest level of quality, the MHTP Partners have made significant investments in our technology capabilities that operate within a unified strategy, governance and quality framework. Several platforms have already achieved NATA accredited promoting shared research and clinical utilisation. Access is available via a collaborative or fee-for-service model throughout the MHTP as well as to national scientific communities.
Creating seamless interaction between use of technology platforms, research and clinical trials capabilities, our Technology Platforms are co-located on a central floor within our new $84M Translational Research Facility (TRF). This not only makes them easily accessible, but also enables innovative approaches to research that integrate knowledge generated from multiple state-of-the-art technologies.
Cell therapies and regenerative medicine
The Cell Therapy and Regenerative Medicine Platform hosts a biological Isolator that provides a fully contained environment suitable for the processing of human cells and tissue. This facility has been established to boost the clinical development of human cell-based therapies and regenerative medicine applications.
The Platform provides a flexible, cost-effective alternative to traditional cell therapy manufacturing and regenerative medicine facilities. It is available to researchers and commercial entities from within and outside the MHTP seeking to progress cell and tissue based therapies to the clinic. The platform houses the BioSpherix Xvivo ISO5 Isolator, an enclosed, integrated and fully controlled environment for cell and tissue processing.
Contact us
Dr Gordon McPhee | Facility Manager
Cell Therapy and Regenerative Medicine Platform
Hudson Institute of Medical Research | Translational Research Facility
t: +61 3 8572 2802
e: gordon.mcphee@hudson.org.au
Dr Mirja Krause | Senior Quality Manager
Cell Therapy and Regenerative Medicine Platform
Hudson Institute of Medical Research | Translational Research Facility
e: mirja.krause@hudson.org.au
Flow cytometry
The Flow Cytometry Facility uses sophisticated instruments for cellular and particle analysis based upon the use of fluorescent probes and markers.
Flow Cytometry is a high speed laser based technology enabling users to generate multiparametric data about the size and shape of the proteins associated with cells or particles of interest. It is a powerful tool in many fields including immunology, stem cell science and cancer.
The facility currently operates one high speed sorter and two cell analysers and provides the following services on a fee-for-service model
- Cell sorting
- Cell analysis
- Training for independent use of cell analysers
- Advice on experimental design and data analysis
Contact us
Michael Thomson | Platform Manager
t:+ 61 3 8572 2494
e: michael.thomson@hudson.org.au
Histology
Histology involves the study of the microscopic anatomy of cells and tissues. Tissues are carefully processed, sectioned and stained revealing delicate morphological features that can indicate health or disease for research, education or clinical studies.
The Histology Platform has two service models
- A full ‘professional’ end-to-end service provided by specialist staff
- A ‘do-it-yourself’ service providing access to laboratory facilities, specialist equipment and consumables.
In addition, the experienced Histology team can provide advice, training and research assistance in
- Dissection
- Cassetting
- Processing – to resin or paraffin
- Cutting – fresh, resin or paraffin sections
- Staining and coverslipping -automated or manual
- Immunohistochemistry – automated utilising the Dako Autostainer
- Micro Tissue array slide supply – creation of micro tissue arrays to suit your needs. We can create the multi block and provide sections for use as control tissue
- Slide scanning – Olympus VS120 for brightfield and fluorescent scanning
Contact us
Camilla Cohen | Monash Histology Platform Manager
Monash University
t: + 61 3 9905 2740
e: camilla.cohen@monash.edu
Angela Vais | MHP-MHTP Histology Node Senior Officer
Hudson Institute
t: +61 3 9594 7442
e: angela.vais@monash.edu
e: histology@hudson.org.au
Micro imaging
The Micro Imaging Facility specialises in optical, fluorescence 4D, live cell and statistical microscopy methodologies, as well as image and data informatics to understand cellular and molecular processes.
MMI-MHTP charges subsidised hourly rates to cover training and instrument access for internal users.
Micro Imaging provides
- Experimental design and troubleshooting
- Instrumentation
- Training
- Research collaboration
- Project development.
Contact us
Dr Sarah Creed | Platform Manager
t: +61 3 8572 2816
e: sarah.creed@hudson.org.au
Proteomics
Hudson Institute accesses world class specialist mass spectrometry and proteomic equipment and expertise though a subscription with the Bio21 Proteomics Facility.
The facility currently has the following mass spectrometers
- 2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
- 2 x Thermo NanoLC/ Q Exactive Plus mass spectrometer
- Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
- Thermo Exactive Focus
- Agilent 6520 LC/ESI-TOF mass spectrometer
- Waters Vion IMS QTOF mass spectrometer
- Waters Synapt HDX G2-Si Ion mobility mass spectrometer
- Shimadzu 9030 QTOF LCMSMS
- 4 x Agilent HPLC systems for sample preparation.
- 3 x Shimadzu Prep HPLC with UV and MS detection
- Waters M Class HPLC for micro-purification
This equipment and downstream analysis can be used to perform common and more complex methods such as
- In-solution protein identification and IP-MS identification
- In-gel protein identification
- Characterise intact recombinant proteins by mass
- Confirm chemical synthesis
- Identify phosphorylation sites
- Immune-precipitation of protein complexes
- Labelled and label-free quantitation
- Stable Isotope Labelling by/with Amino acids in Cell culture
- APEX biotin labelling
- Hydrogen Deuterium Exchange
Contact us
Dr Daniel Gough
e: daniel.gough@hudson.org.au
Dr Daniel Garama
Proteomics Specialist
e: daniel.garama@hudson.org.au
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