Dr Terry Lim holds joint appointments, as a Senior Research Scientist in Dr Pouya Faridi’s laboratory, and in the Monash Proteomics and Metabolomics Platform (MPMP) – Clinical Proteomics Node. He has an Honorary Research Appointment at Hudson Institute. With a major interest in precision oncology, Dr Lim leverages on his expertise in cutting-edge proteomics, phosphoproteomics and immunopeptidomics to advance and accelerate translational and clinical research in order to bring cure closer to the patients.

Dr Lim’s academic journey includes the successful completion of a Bachelor of Biotechnology degree with first-class Honours, with a specialisation in medical biotechnology. Subsequently, under the mentorship of Professor Anthony Purcell, Dr Lim pursued and obtained a PhD from Monash University, where he employed innovative mass spectrometry techniques in the field of proteomics and immunopeptidomics to gain valuable insights into the immunological aspects of the human leukocyte antigen (HLA)-B27 and its correlation with the development of Ankylosing Spondylitis, an autoimmune disease.

Driven by a deep interest in cancer biology, Dr Lim embarked on postdoctoral research in the laboratory of Professor Roger Daly. In this role, he focused on utilising patient derived tumour samples in the form of organoids (PDO) and xenografts (PDX). By employing phosphoproteomics techniques, Dr Lim aimed to unravel the intricate molecular changes occurring in pancreatic, brain, and breast cancer in order to identify novel biomarkers and therapeutic targets for personalised cancer treatments.

Selected publications

• Lim Kam Sian TCC, Goncalves G, Steele JR, Shamekhi T, Bramberger L, Jin D, Shahbazy M, Purcell AW, Ramarathinam S, Stoychev S, Faridi P (2023) SAPrIm, a semi-automated protocol for mid-throughput immunopeptidomics. Front Immunol, 14:1107576.

• Clark KC, Nguyen EV, Niranjan B, Wu Y, Lim Kam Sian TCC, Horvath LG, Taylor RA, Daly RJ (2023) Cell-Type-Specific Signalling Networks Impacted by Prostate Epithelial-Stromal Intercellular Communication. Cancers (Basel), 15:699.

• Yang X, Cruz MI, Nguyen EV, Huang C, Schittenhelm RB, Luu J, Cowley KJ, Shin SY, Nguyen LK, Lim Kam Sian TCC, Clark KC, Simpson KJ, Ma X, Daly RJ (2023) The pseudokinase NRBP1 activates Rac1/Cdc42 via P-Rex1 to drive oncogenic signalling in triple-negative breast cancer. Oncogene, 42:833-847.

• Dannappel MV, Zhu D, Sun X, Chua HK, Poppelaars M, Suehiro M, Khadka S, Lim Kam Sian TC, Sooraj D, Loi M, Gao H, Croagh D, Daly RJ, Faridi P, Boyer TG, Firestein R (2022) CDK8 and CDK19 regulate intestinal differentiation and homeostasis via the chromatin remodeling complex SWI/SNF. J Clin Invest, 132:e158593.

• de Castro Ferezin C, Lim Kam Sian TCC, Wu Y, Ma X, Chüeh AC, Huang C, Schittenhelm RB, Kobarg J, Daly RJ (2022) Identification of biological pathways and processes regulated by NEK5 in breast epithelial cells via an integrated proteomic approach. Cell Commun Signal, 20:197.

• Chew NJ, Lim Kam Sian TCC, Nguyen EV, Shin SY, Yang J, Hui MN, Deng N, McLean CA, Welm AL, Lim E, Gregory P, Nottle T, Lang T, Vereker M, Richardson G, Kerr G, Micati D, Jardé T, Abud HE, Lee RS, Swarbrick A, Daly RJ (2021) Evaluation of FGFR targeting in breast cancer through interrogation of patient-derived models. Breast Cancer Res, 23(1):82.

• Lim Kam Sian TCC, Chüeh AC, Daly RJ (2021) Proteomics-based interrogation of the kinome and its implications for precision oncology. Proteomics, 21:e2000161.

• Faridi P, Woods K, Ostrouska S, Deceneux C, Aranha R, Duscharla D, Wong SQ, Chen W, Ramarathinam SH, Lim Kam Sian TCC, Croft NP, Li C, Ayala R, Cebon JS, Purcell AW, Schittenhelm RB, Behren A (2020) Spliced Peptides and Cytokine-Driven Changes in the Immunopeptidome of Melanoma. Cancer Immunol Res, 8:1322-1334.

• Pandey K, Mifsud NA, Lim Kam Sian TCC, Ayala R, Ternette N, Ramarathinam SH, Purcell AW (2020) In-depth mining of the immunopeptidome of an acute myeloid leukemia cell line using complementary ligand enrichment and data acquisition strategies. Mol Immunol, 123:7-17.

• Lim Kam Sian TCC, Indumathy S, Halim H, Greule A, Cryle MJ, Bowness P, Rossjohn J, Gras S, Purcell AW, Schittenhelm RB (2019) Allelic association with ankylosing spondylitis fails to correlate with human leukocyte antigen B27 homodimer formation. J Biol Chem, 294:20185-20195.

• Schittenhelm RB, Sivaneswaran S, Lim Kam Sian TC, Croft NP, Purcell AW (2016) Human Leukocyte Antigen (HLA) B27 Allotype-Specific Binding and Candidate Arthritogenic Peptides Revealed through Heuristic Clustering of Data-independent Acquisition Mass Spectrometry (DIA-MS) Data. Mol Cell Proteomics, 15:1867-1876.

• Schittenhelm RB, Sian TC, Wilmann PG, Dudek NL, Purcell AW (2015) Revisiting the arthritogenic peptide theory: quantitative not qualitative changes in the peptide repertoire of HLA-B27 allotypes. Arthritis Rheumatol, 67:702-713.